发明名称 |
BACTERIAL ISOLATES OF THE GENUS KLEBSIELLA, AND AN ISOMALTULOSE SYNTHASE GENE ISOLATED THEREFROM |
摘要 |
Two strains of a novel bacterial species, Klebsiella singaporensis LX3 and LX21, are claimed. A nucleotide sequence (kis) encoding a novel form of isomaltulose synthase, KIS, is also claimed. Also claimed is a method for production of isomaltulose in a plant, which method comprises introducing into a cell of such plant a nucleic acid sequence which encodes an enzyme which converts sucrose into isomaltulose in a manner which allows said cell to express said nucleic acid sequence, as well as a functional cloning method of isolating nucleotide sequence encoding the KIS protein comprising the steps of (a) preparing a gene bank from a donor organism that contains a DNA sequence coding for an isomaltulose biosynthesis activity in a suitable host organism, (b) screening the clones of interest from the gene bank by their enhanced reducing sugar content, and (c) isolating the clones which contain a DNA coding for a protein with isomaltulose biosynthesis activity. |
申请公布号 |
EP1257638(B1) |
申请公布日期 |
2007.08.01 |
申请号 |
EP20000906861 |
申请日期 |
2000.02.15 |
申请人 |
AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH |
发明人 |
ZHANG, LIAN, HUI;LI, XIANZHEN;ZHANG, DAOHAI |
分类号 |
A01H1/00;C12N9/90;C12N1/21;C12N15/00;C12N15/09;C12P19/12;C12P19/24;C12R1/22;C12R1/91 |
主分类号 |
A01H1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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