发明名称 |
MICROARRAY-BASED SINGLE NUCLEOTIDE POLYMORPHISM, SEQUENCING, AND GENE EXPRESSION ASSAY METHOD |
摘要 |
There is disclosed a microarray-based single nucleotide polymorphism, sequencing, and gene expression assay method. Specifically, there is disclosed a method using a microarray device wherein a plurality of hybridized structures is formed by contacting the microarray under a hybridizing condition to a hybridizing solution comprising a plurality of tagged targets and a plurality of detection sequences. The detection sequences of each hybridized structure is extended using an extension-ligation solution and an extension-ligation condition. After extension, ligation of the extended sequence occurs to a probe if the terminal nucleotide of a probe is complementary to the hybridized tagged targets. Non-bound material is removed by using a washing solution and a washing method. The target nucleotide and the target sequence of the tagged targets is determined by which probe is ligated to the detection sequences.
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申请公布号 |
WO2006113931(A3) |
申请公布日期 |
2007.07.26 |
申请号 |
WO2006US15323 |
申请日期 |
2006.04.20 |
申请人 |
COMBIMATRIX CORPORATION;LODES, MICHAEL, J.;SUCIU, DOMINIC;MCSHEA, ANDREW |
发明人 |
LODES, MICHAEL, J.;SUCIU, DOMINIC;MCSHEA, ANDREW |
分类号 |
C12Q1/68;C07H21/00;C07H21/04;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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