METHOD OF EFFICIENTLY SCREENING PROTEIN CAPABLE OF SPECIFIC BINDING TO LIGAND

摘要

The present invention relates to a method of selecting a protein specifically binding to a specific ligand. A method of selecting from a diversifying library a protein specifically binding to a ligand of interest, which comprises: a step of allowing proteins existing in the above library to come into contact with the ligand of interest, so as to select proteins binding to the ligand of interest; a step of allowing the obtained proteins to come into contact with a control ligand, so as to determine the presence or absence of the binding ability of the above proteins to the control ligand; and a step of selecting proteins that are determined not to have the binding ability to the control ligand.