| 摘要 |
Provided is an aspartyl-tRNA promoter which is able to strongly express a foreign gene in chlorella and is derived from Chlorella virus SS-2 isolated from fresh water at Seosan area so as to a highly concentrated recombinant protein in the chlorella and plants. The aspartyl-tRNA promoter isolated from an early expression gene derived from Chlorella virus SS-2 has SEQ ID : NO. 1. The transformed microorganism is characterized in that it is obtained by introducing a recombinant vector including the promoter into at least one host selected from the group consisting of bacteria, fungi, and yeast. The microalga is transformed by the recombinant vector including the promoter. The microalga is Nannochloropsis, Tetraselmis, Cheaoceros, Isochryosis, Pavlova, Phaeodactylum, Skeletonama, Navicula, Calonase and Chlorella such as Chlorella ellipsoidea, Chlorella sorokiniana, and Chlorella vulgaris Spirulina. The method for preparing a target protein comprises a step of culturing the transformed microalga.
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