METHOD FOR ESTABLISHING AND PROLIFERATING HUMAN ESC BY CO-CULTURING WITH ALLOGENEIC FEEDER CELLS IN SERUM-FREE MEDIA

摘要

Described herein is the establishment of a human embryonic stem cell line designated Endeavour- 1 under serum free and substantially allogeneic conditions. Also described is the production of 4 clonal cell lines, designated E1C1, E1C2, E1C3 and E1C4, which are derived from Endeavour-1. Each of these cells lines demonstrates pluripotency and long-term self renewal, and the ability to be directed to differentiate into cells exhibiting characteristics of any one or more of ectoderm, mesoderm and endoderm. Also described are cells derived from these cell lines, differentiated cells derived from these lines and uses thereof, Also described are methods of establishing and proliferating a human embryonic stem cells line in an undifferentiated form under serum-free and substantially allogeneic conditions.