发明名称 |
High fidelity detection of nucleic acid differences by ligase detection reaction |
摘要 |
Ligase detection reaction is utilized to distinguish minority template in the presence of an excess of normal template with a thermostable ligase. This process can be carried out with a mutant ligase, thermostable ligase, or a modified oligonucleotide probe. This procedure is particularly useful for the detection of cancer-associated mutations. It has the advantage of providing a quantitative measure of the amount or ratio of minority template.
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申请公布号 |
US7244831(B2) |
申请公布日期 |
2007.07.17 |
申请号 |
US20030405882 |
申请日期 |
2003.04.01 |
申请人 |
PURDUE RESEARCH FOUNDATION |
发明人 |
BARANY FRANCIS;LUO JIANYING;KHANNA MARILYN;BERGSTROM DONALD E. |
分类号 |
C07H21/02;C12N15/09;C07H21/00;C07H21/04;C07K14/82;C12N9/00;C12N15/11;C12Q1/68 |
主分类号 |
C07H21/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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