FLUORESCENT INDICATORS OF HYDROGEN PEROXIDE AND METHODS FOR USING SAME
摘要
The present invention provides a novel genetically encoded fluorescent indicator of hydrogen peroxide. The indicator comprises a sensor polypeptide which is responsive to hydrogen peroxide and a circularly permuted fluorescent protein which is operatively inserted into the sensor polypeptide. Interaction of the sensor polypeptide with hydrogen peroxide results in a change in fluorescence of the fluorescent protein. An isolated nucleic acid molecule encoding a hydrogen peroxide fluorescent indicator of the present invention is also provided. Also provided are vectors, expression cassettes, host-cells, stable cell lines, transgenic animals and transgenic plants comprising a nucleic acid of the present invention. Also provided are methods that use a fluorescent protein of the present invention or the nucleic acid encoding it.
申请公布号
WO2007007199(A3)
申请公布日期
2007.07.12
申请号
WO2006IB02621
申请日期
2006.03.23
申请人
EVROGEN, JSC;BELOUSOV, VSEVOLOD V.;LUKYANOV, SERGEY A.;FRADKOV, ARKADY F.
发明人
BELOUSOV, VSEVOLOD V.;LUKYANOV, SERGEY A.;FRADKOV, ARKADY F.