METHOD OF PREPARING CELL FOR HEART TISSUE REGENERATION

摘要

A method for preparing a cell for heart tissue regeneration is provided to optimize engraftment and survival rate of the cell in the heart muscle, and differentiate the cell into the heart muscle, so that myocardial infarction is prevented and treated. The method for preparing the cells for heart tissue regeneration comprises the steps of: (a) supplying immortalization-inhibited bone marrow stem cells; (b) subculturing the bone marrow stem cells while the cells maintain characteristics of the bone marrow stem cell; (c) culturing the bone marrow stem cells in a cardiac specific medium which induces commitment of the bone marrow stem cell into a cardiomyocyte while the cell maintains characteristics of bone marrow mesenchymal stem cell until 80-90% of the bone marrow stem cells are induced into the cardiomyocytes; and (d) collecting the cells of step(c) when the cultured cells show a marker specific to the bone marrow mesenchymal stem cell.