发明名称 |
SYSTEMS AND METHODS FOR PROTEIN PRODUCTION |
摘要 |
The invention relates to systems and methods for producing proteins of interest. The invention employs genetically-engineered animal or plant cells that have modified protein folding or processing capacities. In one aspect, the invention features genetically-engineered cells comprising one or more recombinant expression cassettes which encode (1) a protein of interest and (2) a polypeptide that is functional in the unfolded protein response (UPR) pathway of the cells. Co-expression of the polypeptide significantly increases the yield of the protein of interest in the genetically-engineered cells. In one example, the genetically-engineered cells are animal cells, and the co-expressed polypeptide is a component or modulator of an XBP1- or ATF6- mediated UPR pathway. |
申请公布号 |
EP1784490(A2) |
申请公布日期 |
2007.05.16 |
申请号 |
EP20050807248 |
申请日期 |
2005.09.01 |
申请人 |
WYETH |
发明人 |
GAO, YIJIE;PICHE, NICOLE, M.;GENG, MEI;HERRMANN, STEPHEN, H.;ZHONG, XIAOTIAN;KRIZ, RONALD;LU, ZHIJIAN |
分类号 |
C12N15/82;C12N15/63;C12N15/85 |
主分类号 |
C12N15/82 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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