METHOD TO IDENTIFY MODULATOR OF CELL SIGNALING

摘要

PROBLEM TO BE SOLVED: To provide methods for assessing libraries of candidate modulators of intracellular signaling transmission pathways. SOLUTION: Methods for assessing libraries of candidate modulators of intracellular signaling transmission pathways are provided. The methods assess the ability of candidates from the library to inhibit the binding of peptides which represent participants in the signaling pathways as either a signal-generating protein or its cognate partner binding at a noncatalytic site. Specific peptides useful in this regard have been identified. In one application, modulators of the immune system can be identified by determining the ability of candidate substances to affect the interaction of PKC-βor a fragment thereof with its cognate. The interaction can be measured using binding of the cognate as an index, or can be measured using a physiological response. COPYRIGHT: (C)2007,JPO&INPIT