| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a screening method of stress-inhibiting substance or stress-enhancing substance, a stress determination method, or a stress diagnostic kit that utilizes a novel stress biomarker. <P>SOLUTION: The serum of a rat loaded with stress by 10-hour submersion constraint is tested by two-dimensional electrophoresis. Electrophoretic images of two-dimensional electrophoresis before and after stress loading are compared, and protein, such as creatine kinase isozyme (CK-MM) detected as four kinds of spots which appear at pH 6.0-7.0 and molecular weight 40-55 kD after stress loading, is used as a marker. The stomach is immediately extracted after blood collection, the area of ulcerated part is measured, and the ratio of the area of the ulcerated part to that of the stomach as a whole is taken as the ulcer index (%). The ulcer index of stress-related gastric ulcer by the submersion constraint test was about 20%. <P>COPYRIGHT: (C)2007,JPO&INPIT |
