摘要 |
<p>The invention provides a method for obtaining a first-generation transgenic plant of the genus Cardamine, which method comprises: (i) contacting a floral meristem of a plant of the genua Cardamine with an Agrobacterium cell, which Agrobacterium cell comprises a heterologous polynucleotide; (ii) allowing the plant to set seeds; (iii) harvesting the seeds; (iv) identifying a seed which comprises the heterologous polynucleotide; and (v) regenerating a plant from the seed identified in step (iv). The method may be used in the evaluation of the effects of polynucleotides which are candidates for transfer into crop species.</p> |