MICROSYSTEM SPECTROSCOPY

摘要

The present invention generally relates to systems and methods for acquiring multiple spectra from a sample, for example, a microfluidic sample. In some aspects of the invention, spectra from a series of locations within a sample are recorded and analyzed, and optionally correlated with an image of the sample. The spectra can be obtained by passing a plurality of light beams through a sample, e.g., using an array of microlenses, diffracting the light beams passing through the sample with a transmission grating, before or after passing the light through the sample, and detecting the resulting light, e.g., as an image. The light beams may pass through the sample before or after passing through the transmission grating. The image can appear as an array of discrete spectra, where each spectrum is associated with a region of the sample where a light beam passed through the sample. The spectra can be analyzed to determine information about the region where the light beam passed through the sample. In some cases, the array of microlenses may be movable and/or replaceable with a second array of microlenses. Also, in some instances, the transmission grating may be movable and/or replaceable with a second transmission grating.