RAPID METHODS FOR DETECTING METHYLATION OF A NUCLEIC ACID MOLECULE

摘要

Disclosed are methods for determining the methylation status of a target double­stranded nucleic acid molecule using PCR amplification and capillary electrophoresis. The methods are generally useful in measuring the methylation status of a nucleic acid sample, including a mammalian genomic DNA sample, and may be further specifically applied to detecting changes in methylation status of a nucleic acid that are associated with exposure to a toxic compound or treatment, or that are associate with a disease or disorder.