发明名称 |
METHOD OF INHIBITING FUNCTION OF CYTOSOLIC PROTEIN BY SINGLE CHAIN ANTIBODY AND USE THEREOF |
摘要 |
<p>The invention relates to a method of inhibiting a function of a cytosolic protein by a single chain antibody (scFv). More specifically, it relates to a method of inhibiting the function of a cytosolic protein by using a single chain antibody constructed by removing a signal peptide of one of VH and VL of an anti-cytosolic protein antibody, a method of analyzing the function of a cytosolic protein by using the single chain antibody, a method of producing the single chain antibody which has a characteristic of specifically inhibiting the function of a cytosolic protein and a non-human transgenic animal in which the single chain antibody which has a characteristic of specifically inhibiting the function of a cytosolic protein is expressed and the function of a cytosolic protein is inhibited. It was revealed that a scFv gene (SHL) in which VH and VL of an anti-WASP-EVH1 monoclonal antibody gene were utilized and VH having a leader sequence and VL in which a leader sequence was removed were connected with a linker and a gene (SHL-CL) having a CL sequence were expressed in the cytosol and inhibited the function of a cytosolic protein.</p> |
申请公布号 |
WO2007020965(A1) |
申请公布日期 |
2007.02.22 |
申请号 |
WO2006JP316150 |
申请日期 |
2006.08.17 |
申请人 |
NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES;SEKIKAWA, KENJI;SATO, MITSURU;KUROSAWA, YOSHIKAZU |
发明人 |
SEKIKAWA, KENJI;SATO, MITSURU;KUROSAWA, YOSHIKAZU |
分类号 |
C12N15/09;A01K67/027;A61K39/395;A61P43/00;C07K16/18;C12N5/10;C12P21/08;C12Q1/02;G01N33/53 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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