发明名称 |
METHOD AND KIT FOR ISOLATING PHOSPHORYLATED PEPTIDES |
摘要 |
The invention relates to a method and a kit for the isolation of phosphorylated peptides from complex mixtures using support materials having chelate ligands based on silica and alkaline elution buffers. In preferred embodiments, the method according to the invention enables the isolation of phosphopeptides from complex sample solutions with a reproducibly high yield and at the same time with high purity and it allows efficient ionisation and detection of the isolated phosphopeptides both by MALDI-TOF and also by ESI mass spectrometry without additional requisite method steps, such as, for example, chromatographic desalination of the sample. |
申请公布号 |
EP1749021(A1) |
申请公布日期 |
2007.02.07 |
申请号 |
EP20050739905 |
申请日期 |
2005.04.21 |
申请人 |
MERCK PATENT GMBH |
发明人 |
ANDERS, JONAS;SCHWAEMMLE, ACHIM;ANDRECHT, SVEN;HENDRIKS, ROBERTUS;BOERNER, ANETTE;RUETER, CORA |
分类号 |
C07K1/22 |
主分类号 |
C07K1/22 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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