发明名称 |
METHOD FOR QUANTITATIVE ANALYSIS OF PROLINE HYDROXYLATION-INDUCED INTERACTIONS BETWEEN HIF-1 AND VBC PROTEIN COMPLEX USING FLUORESCENCE POLARIZATION |
摘要 |
The present invention relates to a method for analyzing the interaction between HIF-1 peptide and VBC protein using fluorescence polarization, more precisely, a method for quantitative analysis of formation of HIF-1-VBC protein complex which is composed of the steps of 1) preparing a fluorescent probe by attaching a fluorescein to hydroxyproline containing HIF-1 peptide; 2) reacting the fluorescent probe with VBC protein; and 3) measuring the fluorescence polarization of the above reactant and then comparing the fluorescence polarization with that of the fluorescent probe itself to investigate the changes of fluorescence polarization; a method for screening an inhibitor of the binding of HIF-1 peptide and VBC protein using the above method; and a method for analyzing the activity of prolyl hydroxylase using the above method. The method of the present invention enables simple analysis of the interaction between HIF-1 peptide and VBC protein by observing changes of fluorescence polarization and thus, it can be effectively used for high-speed screening using a well plate.
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申请公布号 |
US2007020718(A1) |
申请公布日期 |
2007.01.25 |
申请号 |
US20050164813 |
申请日期 |
2005.12.06 |
申请人 |
KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY |
发明人 |
YANG EUN GYEONG;CHO HYUN JU;PARK HYUNSUNG |
分类号 |
C12Q1/30 |
主分类号 |
C12Q1/30 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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