发明名称 |
METHOD FOR ISOLATING COLLAGEN FROM BONE TISSUE OF STOCK-FARM ANIMALS |
摘要 |
FIELD: chemical engineering. ^ SUBSTANCE: method involves pretreating bone tissue of stock-farm animals, comminuting it, precipitating the end product and drying it by lyophilizing. The bone tissue is degreased with alcohol-ether mixture. Demineralization 0.5 and HCl is carried out after grinding during 20 h. Non-resolvable matrix is subjected to proteolysis in HCl of pH=2.0 in pepsin presence at 37°C during 18 h. Then it is centrifuged at 40000g. The end product is precipitated from supernatant with ammonium sulfate to 25% saturation and centrifuged at 6000g. The precipitate is lyophilized against distilled water and chromatographically purified using CM-Sephadex at pH=4.8 and dried with lyophilization. ^ EFFECT: low cost collagen production; high purity cosmetic preparation production. ^ 2 dwg |
申请公布号 |
RU2288729(C2) |
申请公布日期 |
2006.12.10 |
申请号 |
RU20030134131 |
申请日期 |
2003.11.24 |
申请人 |
FEDERAL'NOE GOSUDARSTVENNOE UCHREZHDENIE NAUKI "ROSSIJSKIJ NAUCHNYJ TSENTR "VOSSTANOVITEL'NAJA TRAVMATOLOGIJA I ORTOPEDIJA" IMENI AKADEMIKA G.A. ILIZAROVA FEDERAL'NOGO AGENTSTVA PO ZDRAVOOKHRANENIJU I SOTSIAL'NOMU RAZVITIJU" (FGUN "RNTS "VTO" IMENI A |
发明人 |
LUNEVA SVETLANA NIKOLAEVNA;KOVIN'KA MIKHAIL ALEKSANDROVICH;MATVEEVA ELENA LEONIDOVNA;TALASHOVA IRINA ALEKSANDROVNA;NAKOSKIN ALEKSANDR NIKOLAEVICH |
分类号 |
A61K35/36;A61K9/06;A61K35/32;A61K38/39 |
主分类号 |
A61K35/36 |
代理机构 |
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代理人 |
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地址 |
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