Production of terpenes in transgenic plants having glandulous trichomes, comprises introducing expression cassette having polynucleotide coding sequence into cell of the plant, reconstituting plant from the cell and harvesting the terpenes

摘要

A method for the production of terpenes in transgenic plants having glandulous trichomes (e.g. Nicotiana sylvestris), comprising introducing an expression cassette comprising a polynucleotide sequence encoding taxadiene synthase/casbene synthase into a cell of the plant, reconstituting plant from the cell and harvesting the terpenes, is new. A method for the production of terpenes in transgenic plants having glandulous trichomes, comprising introducing an expression cassette comprising a polynucleotide sequence encoding taxadiene synthase/casbene synthase into a cell of the plant, reconstituting plant from the cell and harvesting the terpenes. The transgenic plant is Nicotiana sylvestrisand the sequence encoding taxadiene synthase/casbene synthase, allows the plant to synthesize the terpenes under the control of a specific promoter of the trichomes, where the cassette comprises an inducer sequence. The terpene is harvested by the recovery of the terpene in the trichome exudate. The production of terpene is carried by blocking the expression of endogenous terpene synthases. Independent claims are also included for: (1) a transgenic plant or seed having glandulous trichomes; and (2) a method of harvesting heterologous terpene.