| 摘要 |
FIELD: biotechnology, in particular production of melanin which represents pigment of high antioxidant and anti-tumor activity. ^ SUBSTANCE: claimed method includes providing by selection of yeast strain Nadsoniella nigra which under deep cultivation accumulates up to 25 g/l of yeast biomass after 96 hours from cultivation starting. Melanin is isolated both from yeast biomass (intracellular melanin) and from cultural liquid (extracellular melanin). Intracellular melanin is isolated by extraction with alkali solution followed by melanin deposition with hydrochloric acid. To isolate extracellular melanin cultural liquid is treated with ethanol followed by melanin deposition with hydrochloric acid. ^ EFFECT: method if increased effectiveness. ^ 4 ex |
