<p>A high yield bacterial strain for producing acetoin named as bacillus pumilus XH195 was deposited in a microorganism deposit center in German, the deposit number is DSM 16187. The strain is rod shaped, 1.5µm to 3.0µm in length and 0.6µm to 0.7µm in diameter. The colony color of the bacterial strain is yellow or white. The strain has the typical fatty acid chromatography as bacillus and the physiological biochemistry characters as bacillus pumilus. When this strain is cultured at 37 for 60 hour with shaking the yield of acetoin can be reached to 58.1g/L or 63.0g/L respectively when glucose or sucrose is used as the substrate.</p>