| 摘要 |
Cytotrophoblast stem cells which express HLA-G and HLA class I antigens have been isolated. Preferably, human cytotrophoblast stem cells are derived from human embryonic stem cells, by culturing embryoid bodies and enriching for embryoid bodies expressing high levels of chorionic gonadotrophin. Human cytotrophoblast stem cells may also be isolated by enriching for cytotrophoblast stem cells that express HLA-G and HLA class I antigen. The isolated cytotrophoblast stem cells may be used for tissue engineering, the modulation of tissue rejection in transplantation therapy, the identification of genes associated with cytotrophoblast stem cell differentiation, the preparation of a library of cytotrophoblast stem cell specific gene products, methods of screening agents that modulate the angiogenic effects of endothelial cells, and the production of mixed cell compositions or chimeric cells. |
