РЕКОМБИНАНТНАЯ ПОЛИНУКЛЕОТИДНАЯ ПОСЛЕДОВАТЕЛЬНОСТЬ, ХАРАКТЕРИЗУЮЩАЯ УНИКАЛЬНЫЙ ТРАНСФОРМАЦИОННЫЙ АКТ МЕЖДУ ГЕНЕТИЧЕСКОЙ КОНСТРУКЦИЕЙ, ВКЛЮЧАЮЩЕЙ ГЕН CRYIIIA, И ГЕНОМНОЙ ДНК КАРТОФЕЛЯ СОРТА ЕЛИЗАВЕТА,ЕЕ ПРИМЕНЕНИЕ И СОДЕРЖАЩИЕ ЭТУ ПОСЛЕДОВАТЕЛЬНОСТЬКЛЕТКА, ТРАНСГЕННОЕ РАСТЕНИЕ И ЕГО ПОТОМСТВО

摘要

FIELD: gene engineering, in particular production of transgenic potato with Colorado beetle resistance and biological and food safety. ^ SUBSTANCE: recombinant polynucleotide sequence of general formula X-A, wherein X is part of nucleotide sequence of introduced genetic construct representing in SEQ ID N 1; A is nucleotide sequence of flanking site of genomic DNA of genus Elizaveta potato, representing in SEQ ID N 2 is constructed; plant cell producing of cryIIIa protein and containing recombinant polynucleotide sequence, as well as potato transgenic plant and vegetative generation thereof with Colorado beetle resistance are obtained. Polynucleotide sequence is used in identification of plant cell producing of cryIIIa protein, plant and vegetative generation. ^ EFFECT: new potato genus with Colorado beetle resistance. ^ 6 cl, 6 dwg, 7 tbl, 7 ex