Lawsonia intracellularis cultivation, anti-lawsonia intracellularis vaccines and diagnostic agents

摘要

A method for large scale cultivation and attenuation of L. intracellularis bacteria by inoculating cells with L. intracellularis bacteria to infect the cells, incubating the infected cells in a reduced oxygen concentration and maintaining the infected cells in suspension. Anti- L . intracellularis vaccines are prepared from cultures grown in suspension. Diagnostic agents are also disclosed.