摘要 |
Biochemical assay comprising a) a substrate (16) being capable of binding at least a target analyte and eventually other constituents contained in a biological sample, b) a test zone (18) on the substrate (16) for sample application, c) a non-immobilized conjugate reagent provided in the test zone (18) for labelling the analyte, said conjugate reagent being capable of specific binding to the analyte but remaining unbound to the substrate (16), d) a flow path (24) for transporting a washing liquid (22) through the test zone (18) and washing an excess of unbound conjugate reagent away from the test zone (18), e) whereby the test zone (18) is also a detection area for detecting the labelled analyte.
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