PURIFICATION OF A BULK OF A FACTOR VII POLYPEPTIDE BY FRACTIONATED ELUTION FROM AN ANION-EXCHANGE MATERIAL

摘要

The present invention relates to the purification of a Factor VII polypeptide from a bulk of a Factor VII polypeptide with respect to desirable glycoforms by fractionated elution from an anion-exchange material with an eluting buffer comprising a certain concentration of calcium ions. The present invention renders it possible to enrich a bulk of a Factor VII polypeptide with respect to desirable glycoforms.