Detecting nucleocytoplasmic shuttling, by injecting anti-reporter gene antibody into nucleus or cytoplasm of transformed cell expressing chimeric reporter gene fusion protein and detecting reporter gene

摘要

Detection of transport between the nucleus and cytoplasm in a transformed cell expressing a chimeric reporter gene fusion protein involves injecting an anti-reporter gene antibody into the nucleus or cytoplasm and detecting the reporter gene in the nucleus and cytoplasm. An independent claim is included for a kit containing a transformed cell expressing a chimeric reporter gene fusion protein and an anti-reporter gene antibody.