<p>The present application relates to a process for purification of a bacterial cytolysin comprising the steps of a) growing a culture of cells expressing bacterial cytolysin, b) mechanically breaking the culture of cells to form an extract, c) prefiltering the extract, d) binding soluble aggregated bacterial cytolysin contained in the extract in the presence of detergent to a hydrophobic interaction chromatography material under high salt (preferably 0.6-2M salt) conditions, and e) eluting bacterial cytolysin in the presence of detergent under low salt (preferably 0-0.2M salt) conditions.</p>
申请公布号
WO2006032499(A1)
申请公布日期
2006.03.30
申请号
WO2005EP10258
申请日期
2005.09.20
申请人
GLAXOSMITHKLINE BIOLOGICALS S.A.;BIEMANS, RALPH, LEON;GORAJ, CARINE;MERTENS, EMMANUEL;VANDERCAMMEN, ANNE
发明人
BIEMANS, RALPH, LEON;GORAJ, CARINE;MERTENS, EMMANUEL;VANDERCAMMEN, ANNE