发明名称 |
ПРОДУЦИРОВАНИЕ IL-21 В ПРОКАРИОТИЧЕСКИХ КЛЕТКАХ-ХОЗЯЕВАХ |
摘要 |
The expression vectors and methods using an E. coli expression system for the large scale production of IL-21 are described. The vectors utilize the IL-21 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Using the expression vectors, the IL-21 gene was produced in E. coli to a level of greater than 1 g/L in fed batch fermentation. Also included are OmpT deficient E. coli strains transformed with an IL-21 expression vector. |
申请公布号 |
RU2005117338(A) |
申请公布日期 |
2006.03.20 |
申请号 |
RU20050117338 |
申请日期 |
2003.12.12 |
申请人 |
ЗАЙМОДЖЕНЕТИКС, Инк. (US) |
发明人 |
ЧАН Чунг (US);ЗЭМОСТ Брус Л. (US);КАВЕРТ Даглас К. (US);ЛИУ Хонг Й. (US);ДЕ ДЖОНГ Кэрен С. (US);МАЙЕР Джеффри Д. (US);ХОЛДЕРМЕН Сьюзан Д. (US) |
分类号 |
C12N15/24;C07K14/54;C12N1/21;C12N15/74;C12P21/02 |
主分类号 |
C12N15/24 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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