| 摘要 |
FIELD: chemistry; biochemistry. ^ SUBSTANCE: present invention pertains to biotechnology. Description is given of a phage particle, exposing on its surface, T-cell receptor (TCR), which is a human scTCR. The exposed dTCR polypeptide pair or exposed scTCR polypeptide contains a series of alpha and beta chains of extra-cellular constant lg of the native TCR domain. A disulphide bond joins amino acid residues of the given series of chains of the constant lg domain, where the given disulphide bond is between cysteic residues. Presented is a library of polypeptide pairs of mutated TCR or scTCR polypeptides, exposed on the described phage particle. Nucleic acid, which encodes the described phage particle, is also presented. A method of identifying TCRs is invented. The invention can be used for making various TCR libraries, for identification of high-affinity TCRs. ^ EFFECT: possibility of making various TCR libraries for identification of high-affinity TCRs. ^ 17 cl, 25 ex, 63 dwg, 2 tbl |
