| 摘要 |
<p>Fluorescence-based kinetic transglutaminase measuring activity, comprising treating transglutaminase with a modified peptide substrate, in which a fluorophor is bounded to the peptide and carries a glutamine acid residue in fluorescence quencher containing an amine function over a spacer, so that splitting of fluorescence quencher takes place by transglutaminase activity and measuring the resulting fluorescence of the molecule, is new. Fluorescence-based kinetic transglutaminase measuring activity, comprising treating transglutaminase with a modified peptide substrate, in which a fluorophor is bounded directly to the N-terminal of the peptide and carries a glutamine acid residue in 5th-position of fluorescence quencher containing an amine function over a spacer, which is bounded to the 5th-position under a formation of an amide compound, so that splitting of fluorescence quencher takes place by the activity of transglutaminase and measuring the resulting fluorescence of the remainder molecule. An independent claim is also included for fluorescence-marked peptide substrate for transglutaminase, comprising a fluorophor, which is bounded directly to the N-terminal of the peptide and carries a glutamine acid residue in 5th-position of fluorescence quencher containing an amine function over a spacer that is bounded to the 5th-position under the formation of an amide compound.</p> |
