| 摘要 |
A method is provided for immunologically measuring a subject substance contained in a sample, which comprises the step of forming an antigen-antibody complex of the subject substance and a specifically binding substance capable of specifically binding to the subject substance in the presence of tricarboxylic acid or tricarboxylate and under acidic conditions. A reagent containing tricarboxylic acid or tricarboxylate for use in the method is also provided. The reagent contains a subject substance and a specifically binding substance capable of immunologically and specifically binding to the subject substance. The reagent is prepared so that the subject substance and the specifically binding substance are bound together under acidic conditions to form an antigen-antibody complex.
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