| 摘要 |
In vivo screening methods for identifying inhibitors of RNA polymerase (RNAP) are provided by the present invention. In certain embodiments, methods according to the invention include steps of: (a) expressing a test RNAP in a cell containing a first reporter; (b) expressing a control RNAP in the cell of step (a) that also contains a second different reporter or, alternatively, a different cell that contains the second different reporter, wherein the first and second different reporters distinguish activities of the test and control RNAPs, respectively; (c) contacting the cell or cells of step (b) with a candidate compound; and (d) assaying to obtain a combined signal from the first reporter and second different reporter wherein a unique combined signal identifies the candidate compound as an inhibitor of an RNAP.
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