INSERTION OF FURIN PROTEASE CLEAVAGE SITES IN MEMBRANE PROTEINS AND USES THEREOF

摘要

Cleavage site for the protease furin is inserted between domains of a membrane glycoprotein. Upon cleavage by furin in the trans­-Golgi network, the protein is separated into individual membrane-free domain that retains its native conformation. This protocol can be used to produce virus membrane protein domains for structural analysis and for trials as vaccines.