| 摘要 |
The invention relates to a novel method for producing cDNA from individual cells after microdissection. The inventive method is inexpensive, comprises few steps and can be carried out also by less skilled laboratory personnel. The novel method for producing cDNA from individual cells obviates the time-consuming and risky step of RNA isolation by allowing for lysis and cDNA synthesis to be carried out in the same reaction vessel in a buffer solution which yields reliable contamination-free results. The buffer is composed of NP40, carrier DNA and Super RNAsin(C), as well as dNTPs and cDNA synthesis primers. |
