FLUORESCENT METHOD AND COMPOUNDS TO MONITOR PROTEIN-LIPID BINDING

摘要

The invention refers to compounds according to formula (I), wherein R<l> is independently in position 2', 3', or in both positions of the A ring and is hydroxyl, linear or branched -0-alkyl, wherein the alkyl group has 1-12 carbon atoms, preferably 3-8, more preferably 4 carbon atoms, -O-(alkyl)COOH wherein the alkyl group has 1-12 carbon atoms, preferably 3-8, more preferably 4 carbon atoms, -O-(aryl)COOH, -0-aryl, linear or branched -O-(CH2)m-CH(NH2)-COOH with m = 1-6, preferably m= 2-4, or any combination thereof; R<4> is in position 8', 10', or 1 l' of the D ring of the compound and is H or (CH2)n -CH3 with n = 0-6, COOH, CN, or halogen, R<5> and R<6> independently are ethyl-, methyl-, H, or -(CH2)3 connected to position 8 or 10 of the D ring, and wherein provided that X is O, R<2> is F, Cl, Br, I, H, CN, -CH=CH-alkyl, -C =- C-alkyl, aryl and R<3> is F, Cl, Br, I, CN, -CH=CH-alkyl, -C = C-alkyl, aryl and provided that X is S, R<2> and R<3> independently are F, Cl, Br, I, H, CN, -CH=CH-alkyl, - C=C-alkyl, aryl. The invention further refers to the use of the compounds according to formula (I), wherein R<1>, R<4>, R<5> and R<6> are defined as aforementioned and wherein R<2> and R<3> independently are F, Cl, Br, I, H, CN, -CH=CH-alkyl, -C=-C-alkyl, or aryl, and wherein X is O or S, as environmentally sensitive fluorescent dyes, particularly in an assay for identifying proteinlipid interactions.