Modified beta-lactamases and uses thereof

摘要

A tool that can screen bacteria for beta-lactamases against a panel of various antibiotics is desirable. A biosensor incorporating an indicator molecule into beta-lactamases may achieve this purpose, but it requires that the attached indicator molecule must not impair the binding affinity of the protein to a great extent to provide a higher sensitivity. A modified beta-lactamases with a residue on the Omega-loop or outside the Omega-loop but close to the active site of beta-lactamase being replaced by a reactive residue is developed in this invention.