A METHOD OF PURIFYING PROUROKINASE

摘要

The invention relates to a method of purifying prourokinase, more specifically, to a method of purifying prourokinase by chromatography. The invention provides a method of purifying recombinant products from cultured cells, comprising: Streamline-SP extended bed chromatography, Sephacryl S-200 gel chromatography, p-aminobenzamidine Sepharose Fast Flow affinity chromatography and DEAE-Sepharose Fast Flow anion exchange chromatography. An E step can be added between steps C and D; concentrating prourokinase by cation exchange Streamline-SP fixed bed chromatography.