发明名称 |
SIGNALLING COMPOUNDS FOR USE IN METHODS OF DETECTING HYDROGEN PEROXIDE |
摘要 |
Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of formula is reacted with peroxide. A detectable product compound of the formula Sig - OH or Sig-O is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs. The signaling compound has the formula: wherein B is a boron atom, R 5 and R 6 are independently selected from hydrogen and lower alkyl groups and can be joined together as a straight or branched alkylene chain forming a five or six-membered ring or an arylene ring, and R 13 is independently selected from cyano, imine, carbonyl, thiazole, carbonyl-substitute thiazole and benzothiazole groups or a group wherein Z is C-C double or triple bond or aromatic ring and n is 1 or 2. |
申请公布号 |
EP1594855(A2) |
申请公布日期 |
2005.11.16 |
申请号 |
EP20040711799 |
申请日期 |
2004.02.17 |
申请人 |
LUMIGEN, INC. |
发明人 |
AKHAVAN-TAFTI, HASHEM;EICKHOLT, ROBERT, A.;LAUWERS, KENNETH, S.;HANDLEY, RICHARD, S. |
分类号 |
C07F5/02;C07D305/00;C07D321/00;C12N9/96;C12Q1/26;G01N;G01N21/76;G01N33/53;G01N33/533;G01N33/534;(IPC1-7):C07D305/00 |
主分类号 |
C07F5/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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