Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.
申请公布号
CA2795339(A1)
申请公布日期
2005.10.20
申请号
CA20052795339
申请日期
2005.04.20
申请人
UNIVERSITY OF UTAH RESEARCH FOUNDATION;BIOFIRE DIAGNOSTICS, INC.
发明人
WITTWER, CARL T.;ZHOU, LUMING;DUJOLS, VIRGINIE E.;HOLDEN, JOSEPH A.;WILLMORE-PAYNE, CARLYNN