Methods for promoting homologous recombination

摘要

Nucleosomal polynucleotides promote homologous strand pairing by recombinase. In the absence of superhelical tension, the efficiency of strand pairing with nucleosomal polynucleotides is substantially higher than that with non-nucleosomal nucleic acids. In addition, a recombinase comprising Rad54 and Rad51 associated activity can function cooperatively in the ATP-dependent remodeling of a nucleosomal polynucleotide (i.e., chromatin).