| 摘要 |
FIELD: medicine, drug production. ^ SUBSTANCE: claimed method for production of heterogeneous deoxyribonucleic acid (DNA) sodium salt from animal male gonads and/or sperm thereof includes grinding and homogenization of starting raw materials in detergent solution, solution treatment at elevated temperature with simultaneous stirring, liquid phase separation by filtering and product precipitation with alcohol. Homogenization is carried out in 1-4 % sodium dodecyl sulfate solution in distilled water at 35-60°C with continuous stirring for 1.5-4 h followed by addition of coagulation agent, namely alkali or earth alkali metal permanganate to produce coagulation agent concentration in solution of 0.0005-0.03 M. Obtained solution is stirred at 35-60°C for 25-90 min, than ionic force is increased up to 1-3 M, solution is heated up to 60-62°C and stirred at abovementioned temperature for 1-3 h. Further contaminant precipitate obtained in coagulation process is separated by filtration thought filter having pore size from 1 to 25 mum. Filtered solution of non-fragmented (high molecular) DNA is cooled to 0-20°C, alcohol is added to obtain finished concentration of 27043.5 vol. %, Then obtained solution is agitated and deposited with alcohol preparation is filtered and separated, washed with 96 % ethanol and stored under ethanol. Obtained DNA preparation is dissolved in 0.01-4.5 M NaCl aqueous solution at 50°C or less; obtained solution is passed through high pressure homogenizer under pressure up to 2000 kg/cm2, obtained fragmented DNA is treated with alcohol wherein deposited with alcohol fermented sodium DNA is centrifuged and separated. Separated precipitate is rewashed with alcohol and dried in desiccator at 50°C or less to produce powder. ^ EFFECT: simplified technology for DNA sodium salt production with increased yield without losses of quality. ^ 1 ex, 3 tbl |
