发明名称 IMPROVED METHOD OF ISOLATING NUCLEIC ACIDS
摘要 <p>The present direction is directed to a method and a kit for the isolation of nucleic acid from a sample containing cells or complex biological material, said method, comprising the steps (i) mixing said sample with a suspension comprising a buffer and a solid support, said suspension having a pH value below pH 5.0 characterized in that said buffer causes lysis of said cells or said biological matrial contained in said sample, further characterized in that subsequent to lysis, the nucleic acid is bound to said solid support, (ii) washing the generated nucleic acid/solid support complex with an appropriate washing solution, and (iii) eluting the nucleic acid from said solid support with an appropriate elution solution.</p>
申请公布号 WO2005093065(A1) 申请公布日期 2005.10.06
申请号 WO2005EP02667 申请日期 2005.03.12
申请人 ROCHE DIAGNOSTICS GMBH;F. HOFFMANN-LA ROCHE AG;ACADEMIC MEDICAL CENTER AT THE UNIVERSITY OF AMSTERDAM;BELD, MARCELLINUS 发明人 BELD, MARCELLINUS
分类号 C12N15/10;C07H1/08;(IPC1-7):C12N15/10 主分类号 C12N15/10
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