发明名称 |
Method for albumin purification |
摘要 |
The present invention is a method of purifying recombinant human serum albumin (rHSA) from a solution, which method comprises to subject a cell culture supernatant (CCS) comprising rHSA cation exchange on a bimodal high salt tolerant matrix; hydrophobic interaction chromatography (HIC); anion exchange; and recovering the purified rHSA. The bimodal high salt tolerant cation exchange matrix used enables performing a purification of a cell culture supernatant directly, in the sense that no further dilution thereof is necessary. Due to its high binding capacity, said bimodal cation exchange matrix also allows use of a smaller amount of matrix as compared to a corresponding conventional cation exchanger matrix. Accordingly, the present invention allows substantial savings as regards volumes and consequently operation costs.
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申请公布号 |
US2005214902(A1) |
申请公布日期 |
2005.09.29 |
申请号 |
US20050514536 |
申请日期 |
2005.05.25 |
申请人 |
BELEW MAKONNEN;LI MEI YAN;ZHANG WEI |
发明人 |
BELEW MAKONNEN;LI MEI YAN;ZHANG WEI |
分类号 |
C07K14/765;(IPC1-7):C07K14/765;C12P21/06 |
主分类号 |
C07K14/765 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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