METHODS AND COMPOSITIONS FOR GENERATING AND AMPLIFYING DNA LIBRARIES FOR SENSITIVE DETECTION AND ANALYSIS OF DNA METHYLATION
摘要
The present invention regards a method of amplifying GC-rich molecules, comprising: providing DNA molecules; subjecting the DNA molecules to heat conditions to denature DNA molecules with low GC content but not to denature DNA molecules with high GC content; ligating an adaptor onto the end of the DNA molecules with high GC content to produce adaptor-ligated molecules; and amplifying the adaptor-ligated molecules with high GC content.
申请公布号
CA2559209(A1)
申请公布日期
2005.09.29
申请号
CA20052559209
申请日期
2005.03.03
申请人
RUBICON GENOMICS, INC.
发明人
SUN, TONG;PINTER, JONATHON H.;BRUENING, ERIC E.;TARRIER, BRENDAN J.;M'MWIRICHIA, JOSEPH M.;TESMER, TIM;KURIHARA, TAKAO;MAKAROV, VLADIMIR L.;KAMBEROV, EMMANUEL