DETECTION OF NUCLEIC ACID BY MULTIPLE SEQUENTIAL INVASIVE CLEAVAGE

摘要

PROBLEM TO BE SOLVED: To provide means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. SOLUTION: The means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences are provided. The methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner are also provided. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby the presence of specific nucleic acid sequences or specific variations thereof are indicated. The methods and devices for the separation of nucleic acid molecules based on charge are further provided. The methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region are also provided. The sensitive and specific methods for the detection of nucleic acid derived from various viruses in a sample are further provided. COPYRIGHT: (C)2005,JPO&NCIPI