发明名称 |
Protein fragment complementation assays for the detection of biological or drug interactions |
摘要 |
The present invention describes a method for detecting biomolecular interactions said method comprising: (a) selecting an appropriate reporter molecule selected from the group consisting of a protein, a fluorescent protein, a luminescent protein and a phosphorescent protein; (b) effecting fragmentation of said reporter molecule such that said fragmentation results in reversible loss of reporter function; (c) fusing or attaching fragments of said reporter molecule separately to other molecules; followed by (d) reassociation of said reporter fragments through interactions of the molecules that are fused to said fragments; and (e) detecting said biomolecular interactions by reconstitution of activity of the reporter molecule with the proviso that said protein is not ubiquitin.
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申请公布号 |
US6929916(B2) |
申请公布日期 |
2005.08.16 |
申请号 |
US20020154758 |
申请日期 |
2002.05.24 |
申请人 |
ODYSSEY THERA INC. |
发明人 |
MICHNICK STEPHEN WILLIAM WATSON;PELLETIER JOELLE NINA;REMY INGRID |
分类号 |
A61K48/00;C07K14/435;C12N1/21;C12N15/09;C12N15/10;C12Q1/02;C12Q1/25;C12Q1/68;G01N33/15;G01N33/50;G01N33/542;G01N33/573;G01N33/58;G01N33/68;(IPC1-7):C12Q1/68;C12N15/63;C12N15/11;C07K14/00 |
主分类号 |
A61K48/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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