发明名称 |
Amplification process using pyrophosphate |
摘要 |
A method for conducting a nucleic acid amplification reaction, said method comprising forming an amplification reaction mixture in the presence of sufficient of a pyrophosphate salt to prevent primer extension taking place, digesting said pyrophosphate salt with a pyrophosphatase enzyme (PPase), and subjecting said reaction mixture to conditions such that an amplification reaction may proceed. This can be used as a "hot start" amplification. Particular novel pyrophosphatase enzymes for use in the method are also described and claimed.
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申请公布号 |
NZ528919(A) |
申请公布日期 |
2005.07.29 |
申请号 |
NZ20020528919 |
申请日期 |
2002.04.22 |
申请人 |
THE SECRETARY OF STATE FOR DEFENCE |
发明人 |
CLARK, DUNCAN ROY;VINCENT, SUZANNE PATRICIA |
分类号 |
C12N15/09;C12N9/16;C12N15/54;C12Q1/68;C12R1/01;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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