METHODS FOR THE PROTEIN PRODUCTION AND PURIFICATION OF SOLUBLE RECOMBINANT POLYPEPTIDES

摘要

The present invention relates to process for the production of a soluble polypeptide , such as 11beta-hydroxysteroid dehydrogenase type 1, having at least one ligand binding site, the process comprising (i) providing a host cell comprising a nucleic acid sequence encoding the soluble polypeptide; (ii) culturing the host cell under conditions whereby the polypeptide is produced, wherein the cell culture medium comprises a non-proteinaceous ligand capable of binding to a ligand binding site of the polypeptide; and (iii) recovering said polypeptide.