T7 amplification and cDNA klenow labeling for expression (TAcKLE) analysis with oligonucleotide microarrays

摘要

The present invention relates to a method of producing labelled, single-stranded antisense cDNA from mRNA, especially useful for hybridization to sense-oriented oligonucleotide libraries. In a preferred embodiment, said producing involves the use of the Klenow fragment of E. coli DNA polymerase I. In another preferred embodiment, the method comprises hybridizing the labelled, single-stranded antisense cDNA to microarrays. Therefore, use of the method for microarray applications, such as comparison of samples obtained from healthy and diseased individuals, is envisaged. Furthermore, a kit for performing the method of the invention is provided.